Biogas production from wheat straw: community structure of cellulose-degrading bacteria

Table 1 Summarised clone sequences of cel5 and cel48

Clone	T-RFs	Most closely related microorganism	Identity (%)	Accession number
Cel48
OTU01	249	Acetivibrio cellulolyticus	69.5	ZP_09463651
OTU02^a	328	Clostridium josui	72.9	BAA32430
OTU03	322	Clostridium straminisolvens	79.0	ACV92097
OTU04	168	Ruminococcus champanellensis	61.8	CBL17316
OTU05	352	Ruminococcus albus	46.1	YP_004105715
OTU06	157	Ruminococcus flavefaciens	68.7	ZP_06145360
OTU07	294	Acetivibrio cellulolyticus	74.3	ZP_09463651
OTU08	355	Clostridium straminisolvens	80.0	ACV92097
OTU09	328	Clostridium cellulolyticum	100	YP_002505088
Cel5
OTU01	74	Eubacterium siraeum	49.5	CBK96866
OTU02	173	Flavobacterium johnsoniae	65.7	YP_001193127
OTU03^b	222	Eubacterium cellulosolvens	52.5	ZP_10167476
OTU04^c	228	Marinilabilia salmonicolor	63.8	ZP_11227339
OTU05	380	Flavobacterium johnsoniae	62.9	YP_001193127
OTU06^d	85	Mahella australiensis	60.4	YP_004463133
OTU07	380	Flavobacterium johnsoniae	62.9	YP_001193127
OTU08	81	Echinicola vietnamensis	57.4	YP_007223444

^aMore closely related to uncultured bacterium from sulphate-reducing reactors (identity 81.3%, ACV50351); ^bMore closely related to uncultured bacterium from buffalo rumen (identity 54.3%, ACA61144) ^cMore closely related to uncultured bacterium from human gut (identity 68.3%, ADD61911); ^dMore closely related to uncultured bacterium from biogas digester (identity 100%, AEV59735).

ISSN: 2192-0567