Characterization by length heterogeneity (LH)-PCR of a hydrogen-producing community obtained in dark fermentation using coastal lake sediment as an inoculum

Table 1 Affiliation of the 16S rRNA cloned sequences to accessions reported in the GenBank

Number of clones	Time (h)	GenBank affiliation [accession no.]	Identity (%)	LH amplicon
3, 2	0, 24	C. bifermentans [GenBank:AB618787]	98 to 100	a
1	24	C. bifermentans [GenBank:AB538434]	99	a
2	24	C. ghonii [GenBank:AB542933]	98	b
2, 5	24, 210	C. aurantibutyricum [GenBank:FJ358641]	96	c
2	24	C. acetobutylicum [GenBank:AB595131]	96	c
3	24	C. butyricum [GenBank:AB595129]	99 to 100	d
1	24	C. tertium [GenBank:AB618789]	99	d
1	24	C. perfringens [GenBank:AB627081]	97	e
1	24	C. perfringens [GenBank:EU728702]	98	e

The sequences were obtained from frozen sediment (time 0) and mesophilic continuous dark fermentation cultures after 24 and 210 h. The association to the community profiling (amplicons a to e) obtained by LH-PCR of clones is given.

ISSN: 2192-0567