Figure 1

Aerobic batch cultivation of K. marxianus DSM 5422 without pH adjustment. Aerobic batch cultivation of K. marxianus DSM 5422 in media with various sources of nitrogen without previous pH adjustment. Concentrated and partially demineralized sweet whey was diluted with the same volume of water, supplemented with various sources of nitrogen, and autoclaved; the clear upper phase was supplemented with trace elements and diluted again with water resulting in media with 3.9 g/L sugar; the media were inoculated and cultivated in conical flasks at 250 rpm and 32°C.