Aerobic batch cultivation of
DSM 5422 in stirred bioreactors using (NH
OH. Aerobic batch cultivation of K. marxianus DSM 5422 in a stirred bioreactor using various amounts of (NH4)2SO4 and NH4OH as sources of nitrogen. DW medium with 2 mL/L trace-element solution was supplemented with (NH4)2SO4 or Na2SO4 (white symbols = 0.4 g/L Na2SO4; grey symbols = 1 g/L (NH4)2SO4; black symbols = 2 g/L (NH4)2SO4); the cultivation occurred in an 1-L stirred reactor at 1,200 rpm, 32°C, and aeration with 50 L/h; the pH was controlled to ≥5 with 2 M NH4OH; the given growth rates were derived from measured CO2 data.